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Related Concept Videos

NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

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NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
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2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

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Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
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2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

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Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
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Applications Of NMR In Biology01:25

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

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Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
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NMR Spectroscopy: Spin–Spin Coupling01:08

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The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
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Collaborative development for setup, execution, sharing and analytics of complex NMR experiments.

Alistair G Irvine1, Vadim Slynko2, Yaroslav Nikolaev2

  • 1School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|January 30, 2014
PubMed
Summary
This summary is machine-generated.

This study introduces NMRplus.org, a collaborative web service for optimizing Nuclear Magnetic Resonance (NMR) experiments. It provides a shared database and simulation tools to improve NMR data acquisition and analysis for researchers worldwide.

Keywords:
Collaborative developmentNMR WikiNMR experiment databasePulse program optimisationSignal-to-noise predictionSpin dynamics analysis

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Area of Science:

  • Chemistry
  • Biophysics
  • Structural Biology

Background:

  • Standard Nuclear Magnetic Resonance (NMR) pulse sequences often require optimization for specific experimental conditions and are typically confined to individual laboratories or users.
  • Optimized NMR experiments are frequently not shared, limiting broader scientific advancement and efficient resource utilization.

Purpose of the Study:

  • To introduce a novel web-based service, NMRplus.org, for the collaborative deposition, annotation, and optimization of NMR experiments.
  • To provide a platform for the systematic analysis and simulation of multidimensional NMR experiments to enhance resource allocation for structural biology.

Main Methods:

  • Development of a web application utilizing a Wiki environment for collaborative pulse sequence development and annotation.
  • Implementation of a flexible mechanism for automatic generation of NMR experiments from deposited sequences.
  • Integration of accurate numerical simulation capabilities for multidimensional NMR experiments, including essential auxiliaries like waveforms and decoupling sequences.

Main Results:

  • Establishment of an online database of NMR experiments with systematic sensitivity evaluations.
  • Demonstration of the application's capability to generate NMR experiments and perform accurate numerical simulations.
  • Facilitation of collaborative optimization of pulse sequences through a shared online framework.

Conclusions:

  • NMRplus.org offers a framework for the collective optimization of NMR pulse sequences, benefiting the entire scientific community.
  • The platform enhances the selection of efficient NMR pulse sequences, improving spectrometer time and analysis effort for protein and macromolecule structural studies.