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Two proteins with reverse transcriptase activities associated with hepatitis B virus-like particles.

M R Bavand1, O Laub

  • 1Department of Genetics, Weizmann Institute of Science, Rehovot, Israel.

Journal of Virology
|February 1, 1988
PubMed
Summary
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Hepatitis B virus (HBV) particles contain novel DNA polymerase-reverse transcriptase activity. This finding provides the first direct evidence of reverse transcriptase in HBV, suggesting a unique replication mechanism.

Area of Science:

  • Virology
  • Molecular Biology
  • Hepatology

Background:

  • Hepatitis B virus (HBV), a DNA virus, is thought to replicate via an RNA intermediate, similar to retroviruses.
  • Sequence homology suggests HBV polymerase may possess reverse transcriptase activity, but direct evidence was lacking.

Purpose of the Study:

  • To provide direct evidence for reverse transcriptase activity within hepatitis B virus particles.
  • To characterize the polymerase activities present in HBV-like particles.

Main Methods:

  • Activity gel analysis was employed to detect in situ DNA polymerase activities.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used for protein separation.

Main Results:

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  • HBV-like particles exhibited two major polymerase activities migrating at approximately 90-kDa and 70-kDa.
  • This is the first demonstration of DNA polymerase-reverse transcriptase activity within HBV-like particles.
  • The 70-kDa protein is proposed to be a product of self-cleavage from the 90-kDa precursor.

    • Conclusions:

    • HBV-like particles possess a novel DNA polymerase-reverse transcriptase activity.
    • The findings support a unique replication strategy for HBV, involving reverse transcription.
    • A precursor-product relationship between the 90-kDa and 70-kDa polymerase activities is suggested.