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Related Experiment Video

Updated: May 3, 2026

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Watching single molecules in action.

Jordan Monnet¹, Terence R Strick

¹Jordan Monnet is in the Institut Jacques Monod, CNRS UMR 7592, Université Paris Diderot, Paris, France monnet.jordan@ijm.univ-paris-diderot.fr.

|January 30, 2014

Summary

This summary is machine-generated.

Researchers used fast Fluorescence In Situ Hybridization (fastFISH) imaging to observe the dynamic process of single DNA molecule transcription. This technique allows visualization of the transcription process at the molecular level.

Keywords:

fluorescence in situ hybridization real-time single-molecule transcription unstructured nucleic acid

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Area of Science:

Molecular Biology
Genetics
Biophysics

Background:

Transcription is a fundamental biological process involving the synthesis of RNA from a DNA template.
Understanding transcription dynamics at the single-molecule level is crucial for deciphering gene regulation.
Existing techniques often lack the temporal and spatial resolution to capture rapid molecular events during transcription.

Purpose of the Study:

To develop and apply a novel imaging technique for real-time observation of single DNA molecule transcription.
To visualize and analyze the step-by-step progression of transcription in individual DNA molecules.

Main Methods:

Utilized fast Fluorescence In Situ Hybridization (fastFISH), a high-speed imaging method.
Applied fastFISH to track the dynamics of transcription initiation, elongation, and termination on single DNA molecules.

Main Results:

Successfully visualized the complete transcription cycle of individual DNA molecules using fastFISH.
Observed distinct intermediate steps and dynamic behaviors during the transcription process.

Conclusions:

FastFISH provides unprecedented insights into the real-time kinetics and mechanisms of single-molecule transcription.
This technique opens new avenues for studying complex gene expression processes at the molecular level.