Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Local delivery of rhBMP-2 affects systemic metabolic response during healing of critically-sized segmental defects in a rat model.

Bone·2026

Same author

Endurance exercise elicits temporal and sexual dimorphic multi-omics remodeling of liver metabolism revealed by MoTrPAC.

Cell reports·2026

Same author

Progesterone induces steroidome and lipidome remodeling in a BRCA-resembling mouse model of high-grade serous ovarian cancer.

Translational oncology·2026

Same author

Dynamic remodeling in lipophilic metabolites during Coffea canephora maturation: A lipidomic study.

Food chemistry·2026

Same author

PFOA induced metabolic and immune perturbations in a SARS-2 infection model.

bioRxiv : the preprint server for biology·2026

Same author

A Multimodal Workflow for Spatial Metabolic Neighborhood Mapping in Neural Rosette Cultures.

bioRxiv : the preprint server for biology·2026

Same journal

Reliability of A Vibration-Based Elastography Protocol For Assessing Achilles Tendon Stiffness Across Multiple Joint Angles In Elite Athletes.

Journal of visualized experiments : JoVE·2026

Same journal

Associations of Inflammatory and Coagulation Biomarkers with Kidney Injury Across Chronic and Acute Clinical Settings.

Journal of visualized experiments : JoVE·2026

Same journal

Intelligent Recommender Systems for Chinese Super League Fan Consumption Behavior Prediction.

Journal of visualized experiments : JoVE·2026

Same journal

A Battery of Quantitative Binocular Vision Tests for Adults: Testing Protocols.

Journal of visualized experiments : JoVE·2026

Same journal

Efficacy Analysis of Paiteling in Treating Persistent High-Risk Human Papillomavirus after Cervical Cancer Surgery.

Journal of visualized experiments : JoVE·2026

Same journal

Clinical Efficacy of Tissue-Bone Homeostasis Manipulation on Soft Tissue Balance and Function in Knee Osteoarthritis.

Journal of visualized experiments : JoVE·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 3, 2026

Conducting Miller-Urey Experiments

Conducting Miller-Urey Experiments

Published on: January 21, 2014

Conducting miller-urey experiments.

Eric T Parker¹, James H Cleaves, Aaron S Burton

¹School of Chemistry and Biochemistry, Georgia Institute of Technology.

Journal of Visualized Experiments : Jove

|January 30, 2014

Summary

This summary is machine-generated.

This study details a simplified experimental protocol for the Miller-Urey spark discharge experiment, demonstrating biomolecule synthesis from early Earth conditions. It provides safety guidelines for simulating primitive planetary environments.

More Related Videos

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Published on: November 11, 2008

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Published on: November 27, 2013

Related Experiment Videos

Last Updated: May 3, 2026

Conducting Miller-Urey Experiments

Conducting Miller-Urey Experiments

Published on: January 21, 2014

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Optimization of the Ugi Reaction Using Parallel Synthesis and Automated Liquid Handling

Published on: November 11, 2008

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Millifluidics for Chemical Synthesis and Time-resolved Mechanistic Studies

Published on: November 27, 2013

Area of Science:

Astrobiology
Origin of Life Studies
Experimental Chemistry

Background:

Stanley Miller's 1953 experiment demonstrated the synthesis of biomolecules from inorganic precursors under simulated primordial Earth conditions.
The original Miller-Urey experiment utilized a complex apparatus to replicate the early Earth's atmosphere-ocean system.

Purpose of the Study:

To provide a general experimental protocol for conducting a simplified Miller-Urey type spark discharge experiment.
To outline safety procedures for handling flammable gases and high-voltage electric discharges in a laboratory setting.

Main Methods:

A simplified 3-liter reaction flask apparatus was designed to simulate the primordial Earth's atmosphere-ocean system.
The experiment involved refluxing a mixture of water, hydrogen (H2), methane (CH4), and ammonia (NH3) under continuous electric discharge for one week.

Main Results:

The protocol facilitates the replication of Miller-Urey type experiments for studying the abiotic synthesis of biomolecules.
The described procedures can be adapted for various electric discharge experiments simulating extraterrestrial planetary environments.

Conclusions:

The simplified protocol makes the Miller-Urey experiment more accessible for educational and research purposes.
This work contributes to understanding the chemical processes that may have led to the origin of life on Earth and potentially elsewhere.