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[Expression change of SH2B1, SOCS3, PTP1B and NPY in mice hypothalamus and its relation with obesity]
1Institute of Medical Sciences, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To investigate the expression pattern of adapter protein with a Src-homology 2 domain (SH2B1), the suppressor of cytokine signaling-3 (SOCS3), protein-tyrosine phosphatase 1B (PTP1B) and neturopetide Y (NPY) in obese and normal mice hypothalamus and its relation with serum leptin and insulin levels.
Methods:
The obesity animal model was prepared with healthy C57/bl6 mice. Lee's index and Homeostasis model assessment-insulin resistance (HOMA-IR) were calculated. The mRNA levels of SH2B1, SOCS3, PTP1B and NPY were measured by fluorescent quantitation RT-PCR. The SH2B1 and NPY protein expressions were detected by Western blot.
Results:
Compared with the normal mice of the same age, SH2B1 mRNA expression in the obese mice hypothalamus decreased. SOCS3 and PTP1B mRNA expression increased. Western blot showed that SH2B1 protein expression decreased, while NPY protein expression increased in the obese mice. Linear correlation analysis showed that the serum leptin and fasting insulin levels were negatively correlated with SH2B1mRNA expression and positively correlated with SOCS3 and PTP1B mRNA expression.
Conclusion:
SH2B1, SOCS3, PTP1B and NPY are key factors for obesity development.
Insights
Obesity in mice is linked to decreased adapter protein with a Src-homology 2 domain (SH2B1) and increased suppressor of cytokine signaling-3 (SOCS3), protein-tyrosine phosphatase 1B (PTP1B), and neuropeptide Y (NPY) in the hypothalamus.
Area of Science:
- Molecular biology
- Endocrinology
- Obesity research
Background:
- Obesity is a complex metabolic disorder influenced by genetic and environmental factors.
- Hypothalamic signaling pathways play a crucial role in regulating energy balance and body weight.
- Specific proteins like SH2B1, SOCS3, PTP1B, and NPY are implicated in appetite and metabolism.
Purpose of the Study:
- To investigate the expression patterns of SH2B1, SOCS3, PTP1B, and NPY in the hypothalamus of obese versus normal mice.
- To examine the correlation between these molecular markers and serum leptin and insulin levels in the context of obesity.
Main Methods:
- Establishment of an obesity animal model using C57/bl6 mice.
- Quantification of mRNA levels for SH2B1, SOCS3, PTP1B, and NPY using fluorescent quantitative RT-PCR.
- Detection of SH2B1 and NPY protein expression via Western blot analysis.
Main Results:
- Obese mice exhibited decreased hypothalamic SH2B1 mRNA and protein expression compared to normal mice.
- Obese mice showed increased hypothalamic SOCS3 and PTP1B mRNA expression.
- NPY protein expression was elevated in obese mice, while serum leptin and insulin levels correlated negatively with SH2B1 and positively with SOCS3 and PTP1B mRNA.
Conclusions:
- SH2B1, SOCS3, PTP1B, and NPY are identified as key molecular factors contributing to obesity development.
- Alterations in these signaling molecules within the hypothalamus are associated with metabolic dysregulation in obesity.
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