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Updated: May 3, 2026

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Published on: February 5, 2020
Highly efficient one-pot multienzyme (OPME) synthesis of glycans with fluorous-tag assisted purification
Joel Hwang1, Hai Yu, Hamed Malekan
1Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA. xiichen@ucdavis.edu.
Abstract:
Oligo(ethylene glycol)-linked light fluorous tags have been found to be optimal for conjugating to glycans for both high-yield enzymatic glycosylation reactions using one-pot multienzyme (OPME) systems and quick product purification using fluorous solid-phase extraction (FSPE) cartridges. The combination of OPME glycosylation systems and the FSPE cartridge purification scheme provides a highly effective strategy for facile synthesis and purification of glycans.

