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Updated: May 3, 2026

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
Published on: June 6, 2025
Uninucleate spores of Phycomyces
1Division of Biology, California Institute of Technology, 91109, Pasadena, California, USA.
Abstract:
Under most culture conditions only 0.3% of the vegetative spores of Phycomyces blakesleeanus are uninucleate. On an acidified minimal medium, the uninucleate fraction can be raised up to 4.5% of the spores. The spore population can be fractionated in a gradient under gravity (1xg) yielding fractions that contain over 80% uninucleate spores. These uninucleate spores are fully viable. When the spores to be fractionated are obtained from a heterokaryotic mycelium, the uninucleate fraction produces homokaryotic mycelia.
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