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Related Experiment Video

Updated: May 3, 2026

Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
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Spinach protein factor and chlorophyllase.

W Terpstra1, A C Weijman

  • 1Biophysical Research Group, Institute of Physics, The State University, Bijlhouwerstraat 6, Utrecht, The Netherlands.

Planta
|January 30, 2014
PubMed
Summary
This summary is machine-generated.

Spinach leaves contain a "Spinach Protein Factor" (SPF) chlorophyllase that enhances light sensitivity in chlorophylls. This enzyme, along with a less active form found in endive and spinach, influences bacteriochlorophyll conversion and light sensitivity.

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Area of Science:

  • Biochemistry
  • Plant Physiology

Background:

  • Spinach leaves possess a "Spinach Protein Factor" (SPF) that increases light sensitivity of colloidal chlorophylls.
  • SPF activity correlates with chlorophyllase activity in spinach leaf extracts and isolated particles.

Purpose of the Study:

  • To identify the nature of the Spinach Protein Factor (SPF).
  • To characterize chlorophyllase activities in spinach and endive leaves.
  • To investigate the influence of these enzymes on bacteriochlorophyll conversion and light sensitivity.

Main Methods:

  • Gel filtration on DEAE- and CM-Sephadex for fractionating spinach leaf extracts.
  • Isolation of small particles from spinach leaf homogenates.
  • Spectroscopic analysis of colloidal bacteriochlorophyll conversion.

Main Results:

  • SPF activity in spinach leaves was found to be parallel with chlorophyllase activity, suggesting SPF is a chlorophyllase.
  • A less active chlorophyllase was identified in endive and spinach leaves, competing with SPF for bacteriochlorophyll.
  • Both chlorophyllases converted bacteriochlorophyll to a form with absorption bands shifted to shorter wavelengths (775 nm to 770-766 nm).
  • SPF chlorophyllase enhanced light sensitivity of the converted bacteriochlorophyll, an effect not observed with the less active chlorophyllase.

Conclusions:

  • The "Spinach Protein Factor" is identified as a chlorophyllase.
  • Distinct chlorophyllase activities exist in spinach and endive leaves, with differing effects on bacteriochlorophyll.
  • SPF chlorophyllase plays a significant role in modulating bacteriochlorophyll light sensitivity.