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Comparative assessment between objective and subjective methods in slides stained by immunohistochemistry
Fernando de Andrade Quintanilha Ribeiro1, Celina Siqueira Barbosa Pereira2, Ricardo Junchen Chi3
1Santa Casa de São Paulo, Faculdade de Ciências Médicas, Departamento de Otorrinolaringologia.
Brazilian Journal of Otorhinolaryngology
|January 30, 2014
Summary
Photometry, an objective method, showed moderate reproducibility for assessing middle ear cholesteatoma slides. Subjective evaluation by experienced examiners proved more reliable due to the irregular nature of the stained tissue.
Area of Science:
- Pathology
- Otolaryngology
- Medical Imaging
Background:
- Objective assessment methods are crucial in scientific studies.
- Photometry offers a potential objective approach for evaluating histological slides, including those with immunohistochemical staining.
Purpose of the Study:
- To compare the objective assessment of middle ear cholesteatoma slides using photometry with subjective evaluations.
- To determine the reliability of photometry in analyzing immunohistochemically stained cholesteatoma samples.
Main Methods:
- Fifty-four acquired middle ear cholesteatoma images were analyzed.
- Immunohistochemical staining was performed using anti-TNF-R2 and anti-TGF-α antibodies.
- Digital slide scanning and subsequent photometric analysis were conducted.
Main Results:
- Photometry-based objective assessment was compared to subjective evaluations by three independent examiners.
- Statistical analysis indicated moderate reproducibility (Kappa values 0.41–0.60) for the photometric method.
- The irregular characteristics of the cholesteatoma slides impacted objective evaluation.
Conclusions:
- The irregular nature of immunohistochemically stained middle ear cholesteatoma slides hinders accurate objective evaluation by photometry.
- Subjective assessment by experienced examiners demonstrated greater reliability for these specific samples.
