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Related Experiment Video

Updated: May 3, 2026

Purification of a High Molecular Mass Protein in Streptococcus mutans
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Structural genomics studies of human caries pathogen Streptococcus mutans.

Lanfen Li1, Jie Nan, Dan Li

  • 1State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China, lilf@pku.edu.cn.

Journal of Structural and Functional Genomics
|January 30, 2014
PubMed
Summary

Structural genomics research on Streptococcus mutans, the cause of dental caries, yielded over 100 crystal structures. This study demonstrates a scalable, cost-effective platform for structural biology applicable to various pathogens.

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Area of Science:

  • Structural biology
  • Genomics
  • Microbiology

Background:

  • Streptococcus mutans is the primary cause of human dental caries.
  • Understanding its atomic structure is crucial for developing drug and vaccine targets.

Purpose of the Study:

  • To establish a large-scale, high-efficiency, and low-cost structural genomics platform.
  • To determine atomic-level structures of Streptococcus mutans proteins for drug and vaccine development.

Main Methods:

  • Developed automated high-throughput crystallization equipment and methods.
  • Selected and cloned 1,391 non-membrane protein targets from 1,963 open reading frames.
  • Over-expressed, purified, crystallized, and determined protein structures using X-ray crystallography.

Main Results:

  • Obtained over 200 protein crystals and 100 crystal structures.
  • Established a functional, scalable, and cost-effective structural genomics platform.
  • Demonstrated the applicability of developed techniques to conventional structural biology.

Conclusions:

  • Structural genomics studies can be effectively performed at a moderate laboratory scale.
  • The developed platform and methods are widely applicable to structural biology research.
  • This work provides valuable structural insights into Streptococcus mutans.