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Gene expression analysis in serial liver fine needle aspirates.

S Lejnine1, M J Marton, I-M Wang

  • 1Merck Research Laboratories, Merck & Co., Inc., Upper Gwynedd, PA, USA.

Journal of Viral Hepatitis
|January 31, 2014
PubMed
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Fine needle aspiration (FNA) offers a low-morbidity method for serial liver tissue sampling. A novel normalization strategy accurately quantifies hepatic gene expression from FNA samples, overcoming blood contamination issues.

Area of Science:

  • Hepatology
  • Molecular Biology
  • Biotechnology

Background:

  • Serial hepatic gene expression measurement in humans is challenging due to low-morbidity methods lacking.
  • Hepatic fine needle aspiration (FNA) has lower morbidity than core needle biopsy but is limited by blood contamination, affecting gene expression quantification.

Purpose of the Study:

  • To validate hepatic fine needle aspiration (FNA) as a reliable method for assessing hepatic gene expression.
  • To develop a strategy to overcome blood contamination biases in FNA samples for accurate gene expression analysis.

Main Methods:

  • Simultaneous liver needle biopsies and FNA procedures in 17 chronic hepatitis C patients.
  • Gene expression profiling using Affymetrix microarrays and TaqMan qPCR.
  • Development of a novel normalization strategy using liver-enriched genes to determine liver percentage in FNA samples.
Keywords:
RNARNA amplificationliver samplingmicroarraynucleic acid analysis

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Main Results:

  • Achieved highly consistent gene expression results between needle biopsy and FNA samples (ρ = 0.99, P < 0.0001) for 21 preselected genes.
  • Validated gene expression findings in canine models.
  • Demonstrated accurate gene expression measurements overcoming blood contamination biases.

Conclusions:

  • Hepatic FNA is a reliable method for serial liver tissue sampling.
  • The developed normalization strategy enables accurate hepatic gene expression analysis from FNA samples.
  • This technique holds potential for diverse preclinical and clinical applications requiring serial liver tissue assessment.