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Mutagenicity and genotoxicity of dicapthon insecticide

Recep Liman1

  • 1Faculty of Arts and Sciences, Molecular Biology and Genetics Department, Usak University, 1 Eylül Campus, 64300, Uşak, Turkey, rliman@hotmail.com.

Cytotechnology
|January 31, 2014
PubMed

Insights

Dicapthon exhibits weak mutagenicity in bacterial assays and induces genotoxic effects, including chromosome aberrations and micronuclei, in human cells. It also demonstrates cytotoxic and cytostatic properties in a dose-dependent manner.

Area of Science:

  • Toxicology
  • Genetics
  • Cell Biology

Background:

  • Dicapthon is an organophosphate insecticide.
  • Pesticides can pose risks to human health through mutagenic and genotoxic effects.
  • Assessing the safety of dicapthon is crucial for understanding its environmental and health impacts.

Purpose of the Study:

  • To investigate the mutagenic potential of dicapthon using bacterial reverse mutation assays.
  • To evaluate the genotoxic effects of dicapthon on human peripheral blood lymphocytes in vitro.
  • To determine the cytotoxic and cytostatic effects of dicapthon on human cells.

Main Methods:

  • Bacterial reverse mutation assay in Salmonella typhimurium strains (TA97, TA98, TA100, TA102) with and without metabolic activation (S9 mix).
  • In vitro tests on human peripheral blood lymphocytes, including chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests.
  • Cytotoxicity and cytostatic effects assessed by monitoring mitotic index and nuclear division index.

Main Results:

  • Dicapthon showed weak mutagenicity in Salmonella typhimurium TA98 without S9 mix at doses of 0.1-100 μg/plate.
  • Increased frequency of SCE, CAs, and MN in human lymphocytes was observed dose-dependently.
  • Dicapthon exhibited significant cytotoxic and cytostatic effects, reducing mitotic and nuclear division indices.

Conclusions:

  • Dicapthon possesses mutagenic and genotoxic properties.
  • The compound induces chromosomal damage and affects cell proliferation in human lymphocytes.
  • These findings highlight potential health risks associated with dicapthon exposure.

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