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Site specific properties of carious dentin matrices biomodified with collagen cross-linkers
Ana K Bedran-Russo1, Sachin Karol2, David H Pashley3
1Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Room #551, 801 South Paulina Street, Chicago, IL 60612, USA. bedran@uic.edu
Purpose:
To assess in non-cavitated carious teeth the mechanical properties of dentin matrix by measuring its reduced modulus of elasticity and the effect of dentin biomodification strategies on three dentin matrix zones: caries-affected, apparently normal dentin below caries-affected zone and sound dentin far from carious site.
Methods:
Nano-indentations were performed on dentin matrices of carious molars before and after surface modification using known cross-linking agents (glutaraldehyde, proanthocyanidins from grape seed extract and carbodiimide).
Results:
Statistically significant differences were observed between dentin zones of demineralized dentin prior to surface biomodification (P < 0.05). Following surface modification, there were no statistically significant differences between dentin zones (P < 0.05). An average increase of 30-fold, 2-fold and 2.2-fold of the reduced modulus of elasticity was observed following treatments of the three dentin zones with proanthocyanidin, carbodiimide and glutaraldehyde, respectively.
