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Related Experiment Video

Updated: Dec 25, 2025

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
06:19

Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining

Published on: November 17, 2018

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Imaging of adipose tissue.

Ryan Berry1, Christopher D Church2, Martin T Gericke3

  • 1Department of Molecular, Cell, and Developmental Biology, Yale University School of Medicine, New Haven, Connecticut, USA.

Methods in Enzymology
|February 1, 2014
PubMed
Summary

This study details adipose tissue imaging methods, including bright-field and fluorescent microscopy, to understand white adipose tissue (WAT) and brown adipose tissue (BAT) roles in metabolism. Analyzing adipocyte size and tissue morphology aids in developing strategies against obesity and metabolic diseases.

Keywords:
AdiposeCell profilerConfocalFrozenLineage tracingParaffinWhole mount

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Area of Science:

  • Endocrinology and Metabolism
  • Cell Biology
  • Histology and Imaging Techniques

Background:

  • Adipose tissue, comprising white (WAT) and brown (BAT) depots, regulates lipid metabolism.
  • WAT stores lipids, while BAT burns them for thermogenesis.
  • Understanding adipose tissue function is crucial for combating obesity and metabolic disorders.

Purpose of the Study:

  • To present diverse methods for processing and imaging adipose tissue.
  • To facilitate the study of adipose tissue mass fluctuations in physiological contexts.
  • To provide insights into mechanisms of BAT activation and WAT accumulation for therapeutic development.

Main Methods:

  • Bright-field colorimetric imaging of paraffin-sectioned adipose tissue with automated adipocyte size analysis.
  • Fluorescent imaging of both paraffin and frozen-sectioned adipose tissue.
  • Confocal fluorescent microscopy of whole-mounted adipose tissue.

Main Results:

  • Detailed protocols for various adipose tissue imaging techniques are provided.
  • Example images illustrate results from each imaging protocol.
  • Strengths and limitations of each method are discussed.

Conclusions:

  • Adipose tissue imaging is vital for understanding metabolic regulation and disease.
  • The presented methods offer valuable tools for researchers in endocrinology and cell biology.
  • Accurate imaging and analysis of adipose tissue can inform therapeutic strategies for metabolic pathologies.