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Glucosyltransferase activity in a water extract of maize pollen
1Department of Agronomy, University of Missouri, Columbia.
Planta
|February 1, 2014
Abstract:
Glucosyltransferase activity was extracted from maize pollen in distilled water. The enzymatic reaction required UDP-glucose, mercaptoethanol and Ca(++), and had a pH optimum at 8.2. Either kampferol or quercetin served as a substrate. Michaelis-Menten constants obtained were 0.6×10(-4) M for quercetin and 0.74×10(-3)M for UDP-glucose. Ammonium-sulfate precipitation of the enzyme gave a 4fold purification.
