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Updated: May 3, 2026

A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Published on: January 17, 2018
[Three-dimensional reconstruction of the cell of a green flagellate based on serial section electron microscopy]
1Botanischer Garten München und Botanisches Institut der Universität München, München, Deutschland.
Abstract:
Cells (+gametes) of Chlamydomonas reinhardii were ultrasectioned serially, and electron micrographs were taken of complete cells. On this basis the model of one typical cell was constructed at a magnification of 36000x. The photographs of the model shown in this paper give a good impression of the architecture and organization of this unicellular organism. This is the first time, to our knowledge, that a whole green plant cell has been reconstructed on a uniform scale. The method of the construction is described.
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