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Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Related Experiment Video

Updated: May 3, 2026

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone
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Tips and techniques for processing and sectioning undecalcified murine bone specimens.

Thomas B Bemenderfer1, Jonathan S Harris1, Keith W Condon1

  • 1Indiana University School of Medicine, Indianapolis, IN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2014
PubMed
Summary

This study details the histological preparation of mineralized mouse bones for bone-specific staining. The methods ensure clear visualization of tissue and cellular morphology using light microscopy.

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Area of Science:

  • Histology
  • Biomedical Engineering
  • Materials Science

Background:

  • Mineralized tissue preparation is crucial for visualizing bone morphology.
  • Standard histological techniques involve fixation, dehydration, embedding, sectioning, and staining.
  • Specific stains highlight mineralized components and cellular structures.

Purpose of the Study:

  • To describe a detailed protocol for processing mineralized mouse bones.
  • To enable histological analysis and light microscopy of bone specimens.
  • To optimize bone-specific staining for morphological studies.

Main Methods:

  • Specimens are fixed in 10% neutral-buffered formalin.
  • Dehydration using graded ethanol (EtOH) solutions, optional xylene clearing.
  • Embedding in polymethyl methacrylate (MMA), sectioning to 4-10 μm.
  • Staining with von Kossa, Goldner's trichrome, or alizarin red S stains.

Main Results:

  • Successfully processed mineralized mouse bone specimens.
  • Achieved clear visualization of tissue and cellular morphology.
  • Demonstrated efficacy of bone-specific stains for histological analysis.

Conclusions:

  • The described protocol provides a reliable method for mineralized bone histology.
  • This technique is essential for detailed morphological studies of bone.
  • Accurate tissue preparation is key for understanding bone structure and pathology.