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Whole-mount in situ hybridization on murine skeletogenic tissues

Timothy Rutkowsky1, Deepika Sharma1, Matthew J Hilton1

  • 1University of Rochester Medical Center, Rochester, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 1, 2014
PubMed

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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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