Jove
Visualize
Contact Us

Related Concept Videos

Microtubule Formation01:23

Microtubule Formation

6.3K
Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation...
6.3K
Microtubule Instability02:17

Microtubule Instability

5.0K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
5.0K
Microtubule Instability02:17

Microtubule Instability

5.2K
5.2K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.1K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.1K
Microtubules01:18

Microtubules

7.9K
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
7.9K
Microtubules01:35

Microtubules

74.7K
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
74.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The kinesin Kip2 promotes microtubule growth using a bipartite polymerase module to deliver tubulin to microtubule plus ends.

Cell reports·2025
Same author

The motor domain of the kinesin Kip2 promotes microtubule polymerization at microtubule tips.

The Journal of cell biology·2023
Same author

Self-repair protects microtubules from destruction by molecular motors.

Nature materials·2021
Same author

Effects of α-tubulin acetylation on microtubule structure and stability.

Proceedings of the National Academy of Sciences of the United States of America·2019
Same author

Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.

Science (New York, N.Y.)·2017
Same author

Tubulin acetylation protects long-lived microtubules against mechanical ageing.

Nature cell biology·2017
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 3, 2026

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
10:27

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Published on: August 25, 2009

10.9K

Micropatterning microtubules.

Didier Portran1

  • 1Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Methods in Cell Biology
|February 4, 2014
PubMed
Summary
This summary is machine-generated.

This study presents a novel method for controlling microtubule (MT) orientation and polarity using UV micropatterning and MT microseeds. This technique enables the precise assembly of dynamic MT networks with specific geometries.

Keywords:
AstersMicropatterningMicrotubule antiparallel organizationMicrotubule microseedsMicrotubule parallel organizationMicrotubulesPLL-PEGSilane-PEGSurface passivation

More Related Videos

Pattern Generation for Micropattern Traction Microscopy
09:26

Pattern Generation for Micropattern Traction Microscopy

Published on: February 17, 2022

2.1K
Self-Assembly of Microtubule Tactoids
08:49

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

4.6K

Related Experiment Videos

Last Updated: May 3, 2026

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
10:27

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

Published on: August 25, 2009

10.9K
Pattern Generation for Micropattern Traction Microscopy
09:26

Pattern Generation for Micropattern Traction Microscopy

Published on: February 17, 2022

2.1K
Self-Assembly of Microtubule Tactoids
08:49

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

4.6K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Materials Science

Background:

  • Microtubules (MTs) are crucial cytoskeletal components involved in various cellular processes.
  • Controlling MT organization is essential for understanding cellular functions and developing biomaterials.
  • Existing methods lack precise control over MT orientation and network geometry.

Purpose of the Study:

  • To develop a method for controlling the orientation and polarity of polymerizing microtubules (MTs).
  • To achieve the reconstitution of specific geometries of dynamic MT networks.
  • To provide a versatile platform for MT-based applications.

Main Methods:

  • Utilizing ultraviolet (UV) micropatterning combined with stabilized MT microseeds.
  • Surface passivation using polyethylene glycol (PEG)-based treatments to prevent non-specific protein adsorption.
  • Imprinting adhesive micropatterns via deep UV lithography and photomasks.
  • Adhering MT microseeds to micropatterns to initiate controlled MT polymerization.

Main Results:

  • Demonstrated precise control over MT orientation and polarity.
  • Successfully reconstituted dynamic MT networks with defined geometries.
  • Established a robust protocol for creating patterned MT structures.

Conclusions:

  • The UV micropatterning technique offers a powerful approach for directed microtubule assembly.
  • This method facilitates the creation of complex and organized MT networks.
  • The protocol provides a foundation for advanced applications in cell biology and nanobiotechnology.