Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

8.0K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
8.0K
Fluid Mosaic Model01:19

Fluid Mosaic Model

14.6K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
14.6K
Membrane Domains01:18

Membrane Domains

6.2K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
6.2K
The Fluid Mosaic Model01:34

The Fluid Mosaic Model

157.4K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
157.4K
Membrane Fluidity01:23

Membrane Fluidity

150.1K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
150.1K
Membrane Fluidity01:26

Membrane Fluidity

14.0K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicting Mechanosensitive T Cell Expansion from Cell Spreading.

Advanced healthcare materials·2025
Same author

Taming Variability in T-Cell Mechanosensing.

Cells·2025
Same author

Assaying and classifying T cell function by cell morphology.

BioMedInformatics·2024
Same author

Plasma membrane abundance dictates phagocytic capacity and functional cross-talk in myeloid cells.

Science immunology·2024
Same author

Improving regulatory T cell production through mechanosensing.

Journal of biomedical materials research. Part A·2024
Same author

Author Correction: Mechanically active integrins target lytic secretion at the immune synapse to facilitate cellular cytotoxicity.

Nature communications·2023
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Related Experiment Video

Updated: May 3, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.2K

Micropatterned, multicomponent supported lipid bilayers for cellular systems.

Debjit Dutta1, Lance C Kam1

  • 1Department of Biomedical Engineering, Columbia University, New York, New York, USA.

Methods in Cell Biology
|February 4, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed patterned lipid bilayers to mimic cell membrane interactions for studying juxtacrine signaling. This technology enables new cell-based assays and drug screening platforms.

Keywords:
Lipid bilayersLithographyMicrocontact printingMicrofabricationMicrofluidicsPatterning

More Related Videos

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

16.5K
Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

18.1K

Related Experiment Videos

Last Updated: May 3, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.2K
Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method
09:38

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer SALB Method

Published on: December 1, 2015

16.5K
Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film
08:23

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

18.1K

Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Lipid bilayers are essential cell structures governing cell functions.
  • Substrate-supported lipid bilayers offer in vitro models of natural membranes.
  • Controlling lipid composition and mobility at micro/nanoscales enables new research avenues.

Purpose of the Study:

  • To describe methods for creating multicomponent lipid bilayers.
  • To discuss design considerations for fabricating these systems.
  • To demonstrate their use in studying juxtacrine cell signaling.

Main Methods:

  • Fabrication techniques combining surface patterning with controlled lipid vesicle exposure.
  • Creation of spatially controlled, locally formed lipid bilayers with distinct compositions.
  • Utilizing patterned bilayers to mimic T cell and antigen-presenting cell organization.

Main Results:

  • Successful creation of multicomponent lipid bilayers with spatially controlled composition.
  • Demonstration of a platform to mimic juxtacrine signaling by presenting specific ligands.
  • Exploration of photochemical polymerization for bilayer patterning.

Conclusions:

  • Patterned lipid bilayers provide a powerful platform for investigating membrane interactions.
  • This technology can advance the development of novel cell-based assays.
  • Potential applications include high-throughput drug screening targeting membrane components.