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Updated: Aug 15, 2026

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
Published on: February 26, 2015
Sequence of a cDNA encoding human keratin No 10 selected according to structural homologies of keratins and their
M Y Darmon1, A Sémat, M C Darmon
1Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, Valbonne, France.
Abstract:
We present here the nucleotide sequence of a 1700 bp-long cDNA encoding human epidermal keratin No. 10 (56.5 kDa). cDNA clones of the acidic keratin family were first isolated from a pBR322 human epidermal cDNA library by hybridization with a probe coding for keratin No. 14. Differential hybridization using total cDNA probes prepared from poly(A)+ RNA extracted either from epidermis (which contains keratin No. 10) and from squamous carcinoma or hepatoma cell lines (which do not express keratin No. 10) made possible the selection of clones potentially coding for keratin No. 10. The 1.7 kb sequence exhibits the characteristic features of an acidic keratin with a constant central rod domain and C-terminal variable structures. Moreover, the sequence shows extensive homologies with the cDNA of murine keratin No. 10.
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