Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods to Assess Microbial Communities01:19

Methods to Assess Microbial Communities

60
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
60
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

836
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
Microbiota of the Large Intestine01:27

Microbiota of the Large Intestine

98
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
98
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

705
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
705
Introduction to the Human Microbiota01:22

Introduction to the Human Microbiota

209
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity,...
209
Genomics02:02

Genomics

35.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
35.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same authorSame journal

Harmonized metagenomic signatures of the gut microbiome reveal robust species, functions, and strain links to inflammatory bowel disease.

Gastroenterology·2026
Same author

Circulating imidazole propionate and coronary heart disease risk: interplay between histidine intake, fiber, and gut microbiome.

BMC medicine·2026
Same author

Item recognition is associated with gut microbiota composition in healthy humans.

Learning & memory (Cold Spring Harbor, N.Y.)·2026
Same author

Shotgun Metagenomic Profiling of the Gut Virome in Prodromal and Confirmed Parkinson's Disease.

Annals of neurology·2026
Same author

Long-lasting gut microbiome and fecal metabolome alterations after colorectal adenoma removal and their relationship to colorectal cancer.

Cell host & microbe·2026
Same author

Dietary sulfur amino acids enhance anti-tumor immunity in colon cancer via an NKT cell-XCL1-cDC1 circuit.

Immunity·2026

Related Experiment Video

Updated: May 3, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

29.4K

Meta'omic analytic techniques for studying the intestinal microbiome.

Xochitl C Morgan1, Curtis Huttenhower1

  • 1Department of Biostatistics, Harvard School of Public Health, Boston, Massachusetts; The Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, Massachusetts.

Gastroenterology
|February 4, 2014
PubMed
Summary

Advances in nucleotide sequencing enable comprehensive human microbiome analysis. This allows for identifying gut dysbiosis linked to disease and potential therapeutic targets using advanced bioinformatic tools.

Keywords:
MetagenomicsMetatranscriptomicsMicrobiomeTools

More Related Videos

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

1.4K
Metagenomic Analysis of Silage
08:43

Metagenomic Analysis of Silage

Published on: January 13, 2017

18.7K

Related Experiment Videos

Last Updated: May 3, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

29.4K
A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
09:52

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease

Published on: January 10, 2025

1.4K
Metagenomic Analysis of Silage
08:43

Metagenomic Analysis of Silage

Published on: January 13, 2017

18.7K

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • Nucleotide sequencing is now affordable and essential for human microbiome research.
  • Large-scale projects have characterized the healthy intestinal microbiome's composition and function.
  • Understanding the microbiome aids in analyzing host and microbial DNA/RNA sequences.

Purpose of the Study:

  • To review systems-level models for intestinal microbiome studies.
  • To discuss analytical techniques for determining microbiome functional capabilities.
  • To explore methods for identifying disease-associated dysbiosis.

Main Methods:

  • Utilizing comprehensive microbial community surveys (e.g., MetaHit, Human Microbiome Project).
  • Applying bioinformatic and statistical tools for sequence analysis.
  • Integrating culture-independent sequences with culture-based isolate genomes.
  • Combining sequence data with functional assays like metaproteomics and metabolomics.

Main Results:

  • Established baseline knowledge of the healthy intestinal microbiome.
  • Developed analytical frameworks for detecting microbiome perturbations.
  • Enabled the contextualization of microbial sequences using genomic data.
  • Facilitated integration of multi-omics data for functional insights.

Conclusions:

  • Nucleotide sequencing is a vital tool for human microbiome research.
  • Bioinformatic approaches can identify disease-causing dysbiosis.
  • Systems-level models and multi-omics analyses are crucial for understanding microbiome function in health and disease.