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Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism
Jeeraporn Kansup1, Daisuke Tsugama2, Shenkui Liu3
1Asian Natural Environmental Science Center, The University of Tokyo, Nishitokyo, Tokyo 188-0002, Japan.
Abstract:
Heterotrimeric G proteins (Gα, Gβ and Gγ) have pleiotropic roles in plants, but molecular mechanisms underlying them remain to be elucidated. Here we show that Arabidopsis Gβ (AGB1) interacts with NPH3, a regulator of phototropism. Yeast two-hybrid assays, in vitro pull-down assays and bimolecular fluorescence complementation assays showed that AGB1 and NPH3 physically interact. NPH3-null mutation (nph3) is known to completely abolish hypocotyl phototropism. Loss-of-function mutants of AGB1 (agb1-1 and agb1-2) showed decreased hypocotyl phototropism, and agb1/nph3 double mutants showed no hypocotyl phototropism. These results suggest that AGB1 is involved in the NPH3-mediated regulation of phototropism.
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