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Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
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Arraying the post-translational glycoproteome (PTG).

Ola Blixt1, Ulrika Westerlind2

  • 1Department of Chemistry, Faculty of Science, University of Copenhagen, Thorvalsdsenvej 40, DK-1871 Fredriksberg, Denmark.

Current Opinion in Chemical Biology
|February 4, 2014
PubMed
Summary
This summary is machine-generated.

Glycopeptide microarrays enable high-throughput analysis of complex protein-glycan interactions. This review covers recent advances in synthesis, immobilization, and applications for diverse biological investigations.

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Area of Science:

  • Biochemistry
  • Glycobiology
  • Analytical Chemistry

Background:

  • Glycosylation, a complex protein modification, presents challenges in understanding its biological roles.
  • Synthesizing diverse glycans and glycopeptides is labor-intensive, necessitating efficient analytical methods.

Purpose of the Study:

  • To review significant developments in glycopeptide microarray synthesis, immobilization, and applications.
  • To highlight the utility of microarrays for high-throughput analysis of protein-glycan interactions.

Main Methods:

  • Glycopeptide microarray fabrication techniques.
  • Immobilization strategies for glycans and glycopeptides.
  • High-throughput screening assays utilizing microarrays.

Main Results:

  • Glycopeptide microarrays facilitate rapid and reliable investigation of protein-glycan interactions.
  • Microarrays enable diverse applications including enzyme activity evaluation, epitope mapping, and serodiagnosis.

Conclusions:

  • Glycopeptide microarrays are powerful tools for advancing glycobiology research.
  • Continued development in microarray technology promises further insights into glycosylation's biological significance.