Comparison of two different laser wavelengths' dental bleaching results by photo-Fenton reaction: in vitro study

The purpose of this study was to perform a preliminary in vitro test on the possible use of two different laser wavelengths, 405 and 532 nm, to improve the dental bleaching results. To perform the test, the degradation of a dye, rhodamine B, under the effects of hydrogen peroxide was used. One hundred and twenty vials were divided into four groups of 30 samples each and, while three of them were irradiated with different wavelengths, 365 nm (reference), 405 nm and 532 nm, the fourth was the non-irradiated control group. Each of the four groups was further divided into three subgroups of 10 cuvettes (n = 10) each. The three subgroups included a group with a rhodamine (RH) solution, a rhodamine and hydrogen peroxide (RH + HP) solution and a rhodamine plus hydrogen peroxide and ferrous gluconate (RH + FR) solution. When hydrogen peroxide was present, only UVA irradiation was able to produce significant results, whereas when the photo-Fenton reaction occurred, all the three wavelengths were able to produce a significant degradation of rhodamine B, with better results for longer wavelengths in comparison with short wavelengths. Within the limitations of this in vitro study, the light of the two laser devices, even remaining less effective than UV activation, showed its ability to improve the performance of bleaching agents based on Fenton photocatalysis, whereas when used in combination with hydrogen peroxide only, the 405-nm laser displayed a small effect and the 532-nm laser produced no effects.