The lack of carbon dioxide evolution in maize leaves in the light

Significant carbon dioxide evolution in the light could not be betected in maize leaves held in single-leaf chambers. The leaves that were used remained attached to the plant and were kept at 30° C and with 200 w m(-2) of light incident on the leaf surface. Several experimental techniques were combined in an attempt to detect CO2 evolution from cells within the leaf; these techniques included that of passing air through, rather than over, the leaf, using different levels of O2 and obtaining CO2 response curves.