Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Higher Prevalence of Health-Risk Factors Among US Adults With Unmet Health Care Needs Due to Cost, 2016.

Journal of public health management and practice : JPHMP·2019
Same author

Rapid rehabilitation nursing in postoperative patients with colorectal cancer and quality of life.

Oncology letters·2019
Same author

The Efficacy of General Practitioner Assessment of Cognition in Chinese Elders Aged 80 and Older.

American journal of Alzheimer's disease and other dementias·2019
Same author

Microtubule actin crosslinking factor 1 (MACF1) knockdown inhibits RANKL-induced osteoclastogenesis via Akt/GSK3β/NFATc1 signalling pathway.

Molecular and cellular endocrinology·2019
Same author

Hepatoprotective activity of isostrictiniin from <i>Nymphaea candida</i> on Con A-induced acute liver injury in mice.

Natural product research·2019
Same author

Self-Template Synthesis of Nanoporous VO<sub>2</sub>-Based Films: Localized Surface Plasmon Resonance and Enhanced Optical Performance for Solar Glazing Application.

ACS applied materials & interfaces·2019

Related Experiment Video

Updated: May 3, 2026

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.3K

A tunable fluorescent timer method for imaging spatial-temporal protein dynamics using light-driven photoconvertible

Xinxin Zhu1, Luyuan Zhang, Ya-Ting Kao

  • 1Department of Chemistry, Columbia University, New York, NY 10027, USA.

Journal of Biophotonics
|February 4, 2014
PubMed
Summary

Researchers developed a novel light-driven fluorescent timer to track protein dynamics within cells. This method uses light to measure protein age, offering tunable speed and high spatial-temporal resolution for biological studies.

Keywords:
fluorescence microscopyfluorescent timerlive cell imagingphotoconvertible fluorescent proteinsprotein lifetimeprotein turnover

More Related Videos

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.2K
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

11.5K

Related Experiment Videos

Last Updated: May 3, 2026

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.3K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

1.2K
Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

11.5K

Area of Science:

  • Cellular Biology
  • Biophysics
  • Microscopy

Background:

  • Cellular functions rely on protein behavior in space and time.
  • Fluorescent proteins track protein location, while fluorescent timers reveal spatial-temporal dynamics.
  • Current fluorescent timers depend on chemical maturation processes.

Purpose of the Study:

  • To introduce a novel light-driven timer for measuring relative protein ages at subcellular locations.
  • To provide a new tool for studying coupled spatial-temporal protein dynamics.
  • To offer a flexible method for analyzing temporal hierarchies in biological processes.

Main Methods:

  • Utilizing photoconvertible fluorescent proteins illuminated chronically with low light.
  • Exploiting light intensity to control the timer's speed, independent of oxygen levels.
  • Characterizing the light-driven timer method in vitro and in vivo.
  • Applying the method to image spatiotemporal distributions of proteins with varying lifetimes.

Main Results:

  • Demonstrated a light-driven timer concept for reporting relative protein ages.
  • Showcased optically tunable timing speeds by adjusting laser intensity.
  • Successfully applied the method to visualize protein dynamics in cellular contexts.
  • Achieved high spatial-temporal resolution in imaging protein distributions.

Conclusions:

  • The proposed light-driven timer offers a novel approach to study protein dynamics.
  • This method provides a flexible optical ruler for analyzing spatially ordered processes over time.
  • The technique enhances the study of temporal hierarchies with precise spatial-temporal resolution.