TSG-6 mediates the effect of tendon derived stem cells for rotator cuff healing

Bone marrow stem cells (MSCs) were able to reduce fibrovascular tissues formation via TNF alpha-stimulated gene/protein 6 (6TSG-6) in various animal models. At the same time, tendon-derived stem cells (TDSCs) were able to promote rotator cuff healing; however, the mechanism is still unknown.

To investigate the role of TSG-6 in the treatment of rotator cuff healing with TDSCs.

45 rats underwent unilateral detachment and repair of the supraspinatus tendon. 15 animals received TDSCs in a fibrin glue carrier(Group A), 15 received TSG-6 silenced TDSCs (Group B), and 15 received fibrin glue for control (Group C). Animals were sacrificed at 4 weeks and evaluated for the biomechanical testing. Statistical analysis was performed with an independent t test with significance set at p = 0.05.

The ultimate stress was greater in the TDSCs group (4.91 ± 1.41 N/mm(2)) as compared with the Control group (2.99 ± 1.04 N/mm(2)) (p < 0.05). However, when silent the expression of TSG-6, the TSG-6 silenced group (3.36 ± 0.96 N/mm(2)) showed no benefit over the control group (p = 0.32).

TSG-6 mediates the function of TDSCs to improve the structure and the attachment strength of the healing tendon-bone interface.