Design and optimization of PLGA-based diclofenac loaded nanoparticles

Area of Science:

• Materials Science
• Nanotechnology
• Pharmaceutical Sciences

Background:

• Drug-based nanoparticle formulations offer enhanced bioavailability and reduced side effects.
• Diclofenac, a nonsteroidal anti-inflammatory drug, can cause gastrointestinal and renal side effects.
• Poly(lactide-co-glycolide) (PLGA) nanoparticles are explored for improved drug delivery.

Purpose of the Study:

• To formulate and characterize diclofenac-entrapped poly(lactide-co-glycolide) (PLGA) nanoparticles.
• To evaluate the effect of different stabilizers (PVA and DMAB) and centrifugation speeds on nanoparticle properties.
• To assess particle size, zeta potential, and drug entrapment efficiency.

Main Methods:

• Emulsion-diffusion-evaporation technique was used for nanoparticle formulation.
• Varying concentrations of poly vinyl alcohol (PVA) and didodecyldimethylammonium bromide (DMAB) were employed as stabilizers.
• Nanoparticles were characterized by particle size, zeta potential, and entrapment efficacy after centrifugation at 8,800 or 12,000 rpm.

Main Results:

• Didodecyldimethylammonium bromide (DMAB) formulations yielded the smallest particle size (108 ± 2.1 nm) and highest zeta potential (-27.71 ± 0.6 mV) at specific concentrations and 12,000 rpm centrifugation.
• Poly vinyl alcohol (PVA) formulations resulted in the smallest particle size (92.4 ± 7.6 nm) and highest zeta potential (-11.14 ± 0.5 mV) at specific concentrations and 12,000 rpm centrifugation.
• Drug entrapment efficiencies reached 77.3 ± 3.5% for DMAB and 80.2 ± 1.2% for PVA formulations.

Conclusions:

• Didodecyldimethylammonium bromide (DMAB) is recommended for enhanced nanoparticle stability during formulation.
• Poly(lactide-co-glycolide) (PLGA) based nanoparticle formulations are effective for diclofenac delivery.
• The study demonstrates successful development of diclofenac-loaded PLGA nanoparticles with optimized stabilizer concentrations and processing conditions.