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Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
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Quantitative Fluorescence Assays Using a Self-Powered Paper-Based Microfluidic Device and a Camera-Equipped Cellular

Nicole K Thom1, Gregory G Lewis1, Kimy Yeung1

  • 1Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA; ; Tel: 814 867 2502.

RSC Advances
|February 4, 2014
PubMed
Summary

This study presents a novel point-of-care fluorescence assay using a paper-based microfluidic device and a smartphone. This accessible technology enables sensitive enzyme biomarker detection in resource-limited settings.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Traditional fluorescence assays require specialized, costly equipment, limiting their use in resource-limited environments.
  • There is a need for accessible, portable, and quantitative diagnostic tools for point-of-care applications.
  • Enzyme biomarker detection is crucial for diagnosing various diseases and conditions.

Purpose of the Study:

  • To develop a low-cost, smartphone-based fluorescence assay for point-of-care diagnostics.
  • To design a paper-based microfluidic device integrating a fluidic battery and LED for fluorescence detection.
  • To demonstrate the quantitative measurement of enzyme biomarkers using visible fluorescence captured by a smartphone camera.

Main Methods:

  • A paper-based microfluidic device was fabricated incorporating an internal fluidic battery, LED, and straw cuvette.
  • A small molecule reagent was designed to exhibit a turn-on fluorescence response upon encountering a specific enzyme biomarker.
  • Fluorescence signals were captured using a smartphone camera and quantified via image processing software.

Main Results:

  • The developed assay successfully detected the enzyme β-D-galactosidase with high sensitivity.
  • Quantitative measurements were achieved down to 700 pM levels within a 30-minute assay time.
  • The study characterized key parameters influencing assay sensitivity and reproducibility.

Conclusions:

  • Smartphone-based fluorescence assays using paper microfluidics offer a viable solution for resource-limited diagnostics.
  • This technology enables sensitive and quantitative detection of enzyme biomarkers at the point of care.
  • The described approach provides a versatile platform for developing various accessible diagnostic assays.