[Dexamethasone induces PC12 cell apoptosis by down-regulating glucose uptake]
Xiaoying Liu1, Yanping Wang1, Chen Qiu1
1Institute of Endocrinology, Fujian Medical University Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Summary
Dexamethasone (DEX) treatment reduced PC12 cell viability and induced apoptosis. This effect is linked to decreased glucose uptake and glucose transporter 3 (GLUT-3) protein expression in these neuroendocrine tumor cells.
Area of Science:
- Neuroscience
- Cell Biology
- Endocrinology
Context:
- PC12 cells are a widely used model for neuroendocrine studies.
- Dexamethasone (DEX) is a potent synthetic glucocorticoid with diverse cellular effects.
- Understanding DEX's impact on cellular metabolism and survival is crucial.
Purpose:
- To investigate the effects of dexamethasone (DEX) on rat PC12 chromaffin tumor cells.
- To assess the impact of DEX on cellular viability, apoptosis, and glucose uptake.
- To explore the role of glucose transporter 3 (GLUT-3) in DEX-induced cellular changes.
Summary:
- Dexamethasone (DEX) treatment at 10 and 100 μmol/L significantly decreased PC12 cell viability.
- DEX induced apoptosis, evidenced by DAPI staining, mPTP opening, and caspase activity.
- Glucose uptake and GLUT-3 protein expression were significantly reduced in DEX-treated PC12 cells.
Impact:
- DEX can induce apoptosis in PC12 cells.
- The mechanism involves the inhibition of GLUT-3 protein expression and subsequent reduction in glucose uptake.
- Findings provide insights into the metabolic and apoptotic effects of glucocorticoids in neuroendocrine tumor models.
Related Concept Videos
01:58Cell Specific Gene Expression
13.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.3K
01:16Hormones Regulating Blood Glucose
7.5K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
7.5K