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Advanced oxidation protein product level in children with chronic otitis media with effusion
Hasan Huseyin Balıkcı1, Mustafa Karakaş2, Muhammet Mustafa Gürdal3
1Department of Otorhinolaryngology, Susehri Government Hospital, Sivas, Turkey.
Insights
Advanced oxidation protein products (AOPPs) were elevated in middle ear effusion fluid of children with chronic otitis media with effusion (COME). These findings suggest AOPPs may indicate oxidative stress in COME pathogenesis.
Area of Science:
- Biochemistry
- Otolaryngology
- Pediatrics
Background:
- Chronic otitis media with effusion (COME) is a common pediatric condition with complex etiology.
- Oxidative stress is increasingly implicated in various inflammatory diseases.
Purpose of the Study:
- To investigate the levels of advanced oxidation protein products (AOPPs) in children diagnosed with COME.
- To explore the potential role of AOPPs as biomarkers in COME.
Main Methods:
- A case-control study involving 25 COME patients and 30 healthy children.
- Measurement of AOPP levels in plasma and middle ear effusion fluid using spectrophotometry.
Main Results:
- AOPP levels were significantly higher in the effusion fluid of COME patients compared to their plasma.
- No significant difference in plasma AOPP levels was observed between COME patients and healthy controls.
- Effusion fluid AOPP levels in COME patients were markedly elevated.
Conclusions:
- COME is associated with protein oxidation abnormalities, specifically within the middle ear effusion.
- Elevated AOPPs in effusion fluid suggest a role for oxidative stress in the pathogenesis of COME.
- AOPPs may serve as potential markers for oxidative stress in COME, warranting further investigation.
Objective:
To determine the level of advanced oxidation protein products (AOPPs) in children with chronic otitis media with effusion (COME), in an effort to elucidate the multifactorial etiology of this disease.
Methods:
This study involved 25 COME patients and 30 healthy children (control group) recruited from the Ear, Nose and Throat (ENT) and Pediatric Departments, respectively, of the Haseki Research and Training Hospital. In the COME group, blood samples were collected before a middle ear operation, and middle ear fluid was sampled during the operation. Blood samples were also obtained from the control subjects. AOPP levels in the plasma and effusion fluid were measured by the spectrophotometric method.
Results:
In the COME group, the mean AOPP levels in plasma and effusion fluid were 168.08 μmol/l and 412.75 μmol/l, respectively. In the control group, the mean plasma AOPP level was 141.54 μmol/l. The plasma AOPP levels did not significantly differ between the COME and control groups (p>0.05). In the COME group, however, the effusion fluid AOPP level (412.75 ± 204.54 μmol/l) was significantly higher than the plasma AOPP level (168.08 ± 68.45 μmol/l; p<0.01).
Conclusion:
We found that AOPP levels were elevated in the effusion fluid, but not in the plasma, of COME patients. Thus, COME was associated with protein oxidation abnormalities. Oxidative stress may play a role in the etiopathogenesis of COME, and AOPPs may be used as markers of oxidative stress; however, further studies are required to confirm these findings.
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