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Updated: May 3, 2026

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
Published on: December 26, 2020
Transcriptional control of RfaH on polysialic and colanic acid synthesis by Escherichia coli K92
Nicolás Navasa1, Leandro B Rodríguez-Aparicio1, Miguel Ángel Ferrero1
1Departamento de Biología Molecular, Área de Bioquímica y Biología Molecular, Universidad de León, Campus de Vegazana, 24071 León, Spain.
Abstract:
The transcriptional antiterminator RfaH promotes transcription of long operons encoding surface cell components important for the virulence of Escherichiacoli pathogens. In this paper, we show that RfaH enhanced kps expression for the synthesis of group 2 polysialic acid capsule in E. coli K92. In addition, we demonstrate for the first time that RfaH promotes cps expression for the synthesis of colanic acid, a cell wall component with apparently no role on pathogenicity. Finally, we show a novel RfaH requirement for growth at low temperatures.
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