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Related Experiment Video

Updated: May 3, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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Genome recognition by MYC.

Arianna Sabò1, Bruno Amati

  • 1Center for Genomic Science of IIT@SEMM, Istituto Italiano di Tecnologia, 20139 Milan, Italy.

Cold Spring Harbor Perspectives in Medicine
|February 5, 2014
PubMed
Summary

The MYC/MAX complex binds DNA, preferentially to active promoters. Overexpression in cancer cells causes MYC to bind nearly all active promoters and enhancers, suggesting altered regulatory mechanisms.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Biology

Background:

  • The MYC oncoprotein plays a critical role in cell proliferation and is frequently dysregulated in cancer.
  • MYC forms dimers with MAX to bind specific DNA sequences, known as E-boxes.
  • MYC's DNA-binding activity is crucial for its function in regulating gene expression.

Purpose of the Study:

  • To elucidate the mechanisms governing MYC/MAX DNA binding in both physiological and overexpression conditions.
  • To propose a model for how MYC recognizes and binds to its target DNA elements.
  • To discuss the implications of MYC's binding dynamics for transcriptional control in normal and cancerous cells.

Main Methods:

  • Literature review and synthesis of current knowledge on MYC/MAX function.

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  • Theoretical modeling of protein-DNA interactions and binding site recognition.
  • Analysis of MYC binding patterns in different cellular contexts (physiological vs. overexpression).

    • Main Results:

    • Under physiological conditions, MYC/MAX binds a subset of active promoters.
    • MYC overexpression leads to widespread binding at active promoters and enhancers.
    • Proposed model suggests low-affinity interactions with transcriptional machinery and DNA scanning guide binding, with sequence recognition being non-obligate.

    Conclusions:

    • MYC/MAX binding is influenced by interactions with other cellular components, not solely DNA sequence.
    • Elevated MYC levels result in broader, less specific DNA binding.
    • Differences in binding affinity may distinguish physiological targets from those invaded during overexpression, impacting regulatory control in cancer.