Quantitative measurement of starch in very small amounts of leaf tissue

A specific enzyme method is described for the routine estimation of starch in small quantities (10-30 mg) of dried leaf tissue. A β-glucanase-free preparation of amyloglucosidase is employed to hydrolyse starch to glucose; this is subsequently estimated by the glucose oxidase technique. The method gives result which agree closely with those obtained by a specific iodine-precipitation method.