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Updated: Jul 12, 2026

Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
Detection of alpha 1-antitrypsin from recombinant Escherichia coli lysates utilizing the particle concentration
B J Del Tito1, D W Zabriskie, E J Arcuri
1Department of Biological Process Sciences, SmithKline and French Laboratories, King of Prussia, PA 19406-0939.
Abstract:
A particle concentration fluorescence immunoassay for the quantitative determination of human alpha 1-antitrypsin in Escherichia coli is described. The principle advantages of this system are speed, automation and a high level of sensitivity compared with enzyme assays and Western blot analysis. Two monoclonal antibodies recognizing different epitopes on the protein are used for the detection and quantitation of alpha 1-antitrypsin. This method has a measured range of 30-2500 ng/ml with a mean accuracy of 5.5% and a precision of 5.1%.
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