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Updated: May 3, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
A tuned affinity-based staurosporine probe for in situ profiling of protein kinases
Xiamin Cheng1, Lin Li, Mahesh Uttamchandani
1Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore117543. chmyaosq@nus.edu.sg.
Abstract:
A clickable and cell-permeable affinity-based probe (AfBP) was designed from staurosporine, by incorporating an electrophilic chloroacetamide warhead to facilitate in situ proteome labeling of a range of potential kinase targets.
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