Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Instrument Calibration01:12

Instrument Calibration

1.4K
Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy.
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
1.4K
Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

37.6K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
37.6K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
Glassware Calibration01:11

Glassware Calibration

2.0K
Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes.
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
2.0K
Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

2.1K
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
2.1K
Calibration Curves: Linear Least Squares01:20

Calibration Curves: Linear Least Squares

4.3K
A calibration curve is a plot of the instrument's response against a series of known concentrations of a substance. This curve is used to set the instrument response levels, using the substance and its concentrations as standards. Alternatively, or additionally, an equation is fitted to the calibration curve plot and subsequently used to calculate the unknown concentrations of other samples reliably.
For data that follow a straight line, the standard method for fitting is the linear...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence of SARS-CoV-2 bacteriophage potential in human gut microbiota.

F1000Research·2025
Same author

The first report on detecting SARS-CoV-2 inside bacteria of  the human gut microbiome: A case series on asymptomatic family members and a child with COVID-19.

F1000Research·2024
Same author

Effect of a Phytochemical-Rich Olive-Derived Extract on Anthropometric, Hematological, and Metabolic Parameters.

Nutrients·2024
Same author

Who Is the Intermediate Host of RNA Viruses? A Study Focusing on SARS-CoV-2 and Poliovirus.

Microorganisms·2024
Same author

Correction: Enhanced molecular release from elderly bone samples using collagenase I: insights into fatty acid metabolism alterations.

Journal of translational medicine·2024
Same author

A retrospective cohort study on early antibiotic use in vaccinated and unvaccinated COVID-19 patients.

Journal of medical virology·2024

Related Experiment Video

Updated: May 3, 2026

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

6.7K

PROSAD: a powerful platform for instrument calibration and quantification.

Matteo Floridia1, Simone Cristoni

  • 1Newservice srl, Via Montenapoleone 10, 20121, Milano, Italy.

Rapid Communications in Mass Spectrometry : RCM
|February 6, 2014
PubMed
Summary
This summary is machine-generated.

The PROgressive SAmple Dosage (PROSAD) platform enhances mass spectrometry (MS) by providing dynamic calibration, improving quantitative accuracy and stability in complex samples.

More Related Videos

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

11.8K
Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
10:22

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements

Published on: September 7, 2019

10.8K

Related Experiment Videos

Last Updated: May 3, 2026

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

6.7K
PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
08:43

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

11.8K
Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
10:22

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements

Published on: September 7, 2019

10.8K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Mass spectrometry (MS) quantitative results are limited by instrumental biases.
  • Improved calibration and correction methods are crucial for reliable MS data.
  • The PROgressive SAmple Dosage (PROSAD) platform was developed to address these limitations.

Purpose of the Study:

  • To develop and test the PROSAD platform for enhanced mass spectral quantification.
  • To overcome instrumental response biases in MS analysis.
  • To improve the accuracy and precision of quantitative MS results.

Main Methods:

  • PROSAD integrates optimized sample preparation and data analysis with liquid chromatography/MS (LC/MS) and a low-voltage ionization source.
  • The system utilizes a dedicated mathematical algorithm for dynamic linear calibration.
  • Tested on HCT Ultra ion trap, LTQ XL Orbitrap, and TSQ Vantage triple-stage quadrupole mass spectrometers.

Main Results:

  • PROSAD demonstrated superior quantification compared to traditional static calibration in a leucomalachite green-fish homogenate assay.
  • Accurate quantification of atrazine in tea matrix samples was achieved using PROSAD.
  • The platform provided better quantitative results in both test cases.

Conclusions:

  • PROSAD establishes a dynamically optimized calibration curve for MS data.
  • The platform significantly increases the stability, accuracy, and precision of MS quantification.
  • PROSAD offers a robust solution for reliable quantitative mass spectrometry.