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GC-MS analysis of bio-active compounds in methanolic extract of Lactuca runcinata DC
Lakshmi Kanta Kanthal1, Akalanka Dey2, K Satyavathi3
1Department of Pharmacology, Koringa College of Pharmacy, Tallarevu Mandal, Andhra Pradesh, India.
Background:
The presence of phytochemical constitutes has been reported from species of the Compositae (Asteraceae). Hitherto no reports exist on the phytochemical components and biological activity of Lactuca runcinata DC.
Objective:
The present study was designed to determine the bioactive compounds in the whole plant methanol extract of Lactuca runcinata.
Materials And Methods:
Phytochemical screening of the entire herb of Lactuca runcinata DC revealed the presence of some bio-active components. Gas chromatography-mass spectrometry (GC-MS) analysis of the whole plant methanol extract of Lactuca runcinata was performed on a GC-MS equipment (Thermo Scientific Co.) Thermo GC-TRACE ultra ver.: 5.0, Thermo MS DSQ II.
Results:
The phytochemical tests showed the presence of alkaloids, cardiac glycosides, flavonoids, phenols, phlobatannin, reducing sugars, saponins, steroids, tannins, terpenoids, volatile oils, carbohydrates, and protein/amino acids in methanolic extract of L. runcinata. The GC-MS analysis has shown the presence of different phytochemical compounds in the methanolic extract of Lactuca runcinata. A total of 21 compounds were identified representing 84.49% of total methanolic extract composition.
Conclusion:
From the results, it is evident that Lactuca runcinata contains various phytocomponents and is recommended as a plant of phytopharmaceutical importance.
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