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Analysis of the essential oil components from different Carum copticum L. samples from Iran
Mohammad M Zarshenas1, Soliman Mohammadi Samani2, Peyman Petramfar3
1Department of Traditional Pharmacy, School of Pharmacy, Shiraz, Iran ; Medicinal Plants Processing Research Center, Shiraz, Iran ; Students Research Committee, Shiraz, Iran.
Background:
The family Apiaceae is defined with the diversity of essential oil. Fruits of Ajwain (Carum copticum), a famous herb of Apiaceae, accumulate up to 5% essential oil which is remarked as important natural product for food and flavoring industry, as well as pharmacological approaches. It is believed that differences in essential oil profile in a certain plant are resulted from various cultivation situations and locations, time of cultivation and also different extracting method.
Objective:
Present study aimed to evaluate major components of ten different collected Ajwain samples from random cultivation locations of Iran.
Materials And Methods:
Samples were individually subjected to hydrodistillation using a Clevenger-type apparatus for the extraction of essential oil. GC/MS analysis for samples was carried out using Agilent technologies model 7890A gas chromatograph with a mass detector.
Results:
The yield of extracted essential oil was calculated as 2.2 to 4.8% (v/w) for ten samples. Major oil components were thymol, para-cymene and gamma-terpinene. Five of ten samples have thymol as the main component with amount of 35.04 to 63.31%. On the other hand, for four samples, para-cymene was major with amount of 40.20 to 57.31% and one sample had gamma-terpinene as main constituent containing 37.43% of total oil. Accordingly, three different chemotypes, thymol, para-cymene and gamma-terpinene can be speculated from collected samples.
Conclusion:
While these components possess pharmacological effect, screening of different chemotypes not only represent the effect of cultivation situations and locations but also can be beneficial in further investigation.
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