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Campylobacter pylori in Swedish patients referred for gastroscopy
H Gnarpe1, P Unge, C Blomqvist
1Department of Clinical Bacteriology, Gävle Central Hospital, Sweden.
Abstract:
Campylobacter pylori was isolated more often from patient with peptic ulcers and in an age-related manner in a material of 395 consecutive patients referred for gastroscopy. Direct microscopy was done with Gram and Acridine Orange stain and found too insensitive for practical use. All patients were investigated serologically with an enzyme immunoassay which showed excellent correlation with positive cultures for C. pylori. The findings were discussed, and the enzyme immunoassay, with a negative predictive value of 0.99, was found to be a valuable tool for primary screening of patients suspected of being carriers of C. pylori.
Insights
Campylobacter pylori, a cause of peptic ulcers, was identified in patients undergoing gastroscopy. An enzyme immunoassay proved highly effective for screening C. pylori carriers.
Area of Science:
- Gastroenterology
- Microbiology
- Medical Diagnostics
Background:
- Peptic ulcer disease is often associated with specific bacterial infections.
- Accurate identification of causative agents is crucial for effective treatment.
- Campylobacter pylori (now Helicobacter pylori) was emerging as a significant pathogen.
Purpose of the Study:
- To investigate the prevalence of Campylobacter pylori in patients with peptic ulcers.
- To evaluate diagnostic methods for C. pylori detection.
- To assess the utility of serological testing for C. pylori screening.
Main Methods:
- Analysis of 395 patients undergoing gastroscopy.
- Bacterial isolation and culture.
- Direct microscopy using Gram and Acridine Orange stains.
- Serological investigation using an enzyme immunoassay.
Main Results:
- Campylobacter pylori isolation correlated with peptic ulcer presence and patient age.
- Direct microscopy demonstrated low sensitivity for practical application.
- Enzyme immunoassay showed excellent correlation with positive C. pylori cultures.
- The enzyme immunoassay achieved a negative predictive value of 0.99.
Conclusions:
- Campylobacter pylori is frequently found in patients with peptic ulcers.
- Enzyme immunoassay is a valuable and sensitive tool for primary screening of C. pylori.
- Serological testing offers a reliable method for identifying potential C. pylori carriers.