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Related Experiment Videos

Direct ion channel gating: a new function for intracellular messengers.

P E Hockberger1, D Swandulla

  • 1Department of Molecular Biophysics, AT&T Bell Laboratories, Murray Hill, New Jersey.

Cellular and Molecular Neurobiology
|September 1, 1987
PubMed
Summary

Intracellular messengers directly regulate ion channels without protein kinases. This direct signaling pathway, confirmed by single-channel studies, is increasingly observed in various cell types.

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Area of Science:

  • Cellular signaling
  • Neuroscience
  • Ion channel physiology

Background:

  • Intracellular messengers like calcium (Ca2+), cyclic AMP, cyclic GMP, and inositol-1,4,5-triphosphate (IP3) are traditionally thought to act via protein kinase activation.
  • Protein kinase activation influences membrane ionic conductance, likely through ion channel phosphorylation, particularly in excitable cells.
  • Emerging evidence suggests intracellular messengers can directly modulate ionic conductances, bypassing protein kinase pathways.

Purpose of the Study:

  • To review and examine instances of direct ionic conductance activation by intracellular messengers.
  • To focus on evidence derived from single-channel studies of isolated membrane patches.
  • To highlight the expanding range of cell types exhibiting this direct signaling mechanism.

Main Methods:

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  • Review of existing literature reporting direct effects of intracellular messengers on ionic conductances.
  • Emphasis on studies utilizing single-channel recording techniques on isolated membrane patches.
  • Analysis of data from diverse cell types, including neuronal and nonneuronal origins.

Main Results:

  • Compilation of documented cases where intracellular messengers directly influence ion channel activity.
  • Confirmation that direct modulation occurs independently of protein kinase activation.
  • Identification of a growing list of cell types demonstrating this direct signaling pathway.

Conclusions:

  • Intracellular messengers possess the capability to directly modulate membrane ionic conductances.
  • This direct mechanism represents a significant alternative to the canonical protein kinase-dependent signaling pathway.
  • The phenomenon of direct ionic channel gating by intracellular messengers is widespread and observed across various cell types.