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Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate
1Department of Chemistry, National University of Singapore, Singapore 117543. chmgaoz@nus.edu.sg.
Nanoscale
|February 7, 2014
Abstract:
By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor (FR). A detection limit of 50 fM FR was obtained using UV-vis spectrometry and 10 pM FR could be visualized by the naked eye.
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