High-allelic variability in HLA-C mRNA expression: association with HLA-extended haplotypes
F Bettens1, L Brunet1, J-M Tiercy1
1National Reference Laboratory for Histocompatibility, Transplantation Immunology Unit, Department of Genetics and Laboratory Medicine, University Hospital, Geneva, Switzerland.
Genes and Immunity
|February 7, 2014
Summary
Human Leukocyte Antigen (HLA)-C expression varies greatly between individuals. This study found HLA-C mRNA levels correlate more strongly with extended HLA haplotypes than specific alleles or 5' region polymorphisms.
Area of Science:
- Immunogenetics
- Molecular biology
Background:
- Human Leukocyte Antigen (HLA)-C is crucial for transplantation and HIV control.
- Previous studies linked HLA-C expression to polymorphisms in its 5' and 3' regions.
Purpose of the Study:
- To investigate the correlation between HLA-C mRNA expression and genetic variations.
- To determine if extended HLA haplotypes influence HLA-C expression levels.
Main Methods:
- Real-time PCR was used to determine HLA-C mRNA levels in blood donors.
- Analysis focused on locus- and allele-specific expression and extended HLA haplotypes.
Main Results:
- Significant inter-individual variability in HLA-C mRNA expression was observed.
- HLA-C expression showed lower variability for C*07:01 but higher for C*06:02, C*04:01, and C*03:04 alleles.
- Previously reported associations with specific single nucleotide polymorphisms were not confirmed.
Conclusions:
- HLA-C mRNA expression levels are more closely associated with extended HLA haplotypes.
- Extended haplotypes, rather than individual allotypes or 5'-region polymorphisms, appear to be the primary determinant of HLA-C expression.
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