Complete genome sequence of Dehalobacter restrictus PER-K23(T.)
Thomas Kruse1, Julien Maillard2, Lynne Goodwin3
1Wageningen University, Agrotechnology and Food Sciences, Laboratory of Microbiology, Dreijenplein 10, NL-6703 HB Wageningen, The Netherlands.
Standards in Genomic Sciences
|February 7, 2014
Summary
Dehalobacter restrictus strain PER-K23’s genome was sequenced, revealing 25 reductive dehalogenase genes. This finding suggests a greater capacity for organohalide respiration than previously understood.
Area of Science:
- Microbiology
- Genomics
- Environmental Science
Background:
- Dehalobacter restrictus strain PER-K23 is the type strain for Dehalobacter restrictus.
- This bacterium performs organohalide respiration, utilizing H2 oxidation to dechlorinate chloroethenes.
- No other growth modes have been observed for this strain.
Purpose of the Study:
- To present the first complete genome sequence of a Dehalobacter species.
- To identify genes associated with the metabolic capabilities of Dehalobacter restrictus.
- To explore the potential for organohalide respiration within the Dehalobacter genus.
Main Methods:
- Whole-genome sequencing of Dehalobacter restrictus strain PER-K23.
- Bioinformatic analysis to identify protein-coding and RNA genes.
- Comparative genomics to analyze gene clusters, particularly those encoding reductive dehalogenases.
Main Results:
- The genome of Dehalobacter restrictus PER-K23 is 2,943,336 bp long, containing 2,826 protein-coding and 82 RNA genes.
- The genome includes 5 copies of the 16S rRNA gene.
- A significant finding is the presence of 25 predicted reductive dehalogenase genes, mostly full-length and clustered in two main regions.
Conclusions:
- The genome sequencing provides a foundational resource for understanding Dehalobacter restrictus.
- The high number and organization of reductive dehalogenase genes indicate a substantial capability for organohalide respiration.
- This discovery expands the known metabolic potential of the Dehalobacter genus for bioremediation applications.
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