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Staphylococcus aureus subsp. anaerobius strain ST1464 genome sequence.

Haitham Elbir1, Catherine Robert1, Ti Thien Nguyen1

  • 1Aix Marseille Université, URMITE, Marseille, France.

Standards in Genomic Sciences
|February 7, 2014
PubMed
Summary

Staphylococcus aureus subsp. anaerobius, a cause of Morel's disease, has a unique genome. This genomic analysis reveals its distinct evolutionary path and potential for developing new diagnostic tools.

Keywords:
Morel’s diseaseSOLiDStaphylococcus aureus subsp. anaerobiusgenome

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Area of Science:

  • Microbiology
  • Genomics
  • Bacterial Pathogenesis

Background:

  • Staphylococcus aureus subsp. anaerobius exhibits microaerophilic growth, distinguishing it from other S. aureus strains.
  • This subspecies is implicated in animal Morel's disease and human abscess formation.

Purpose of the Study:

  • To sequence and analyze the genome of Staphylococcus aureus subsp. anaerobius ST1464.
  • To understand the genetic basis of its unique characteristics and evolutionary divergence.

Main Methods:

  • Whole-genome sequencing of Staphylococcus aureus subsp. anaerobius ST1464.
  • Bioinformatic analysis of the genome, including open reading frame identification, phage genome detection, and in-silico multilocus sequence typing.

Main Results:

  • The 2,604,446-bp genome contains 2,660 open reading frames and harbors phage genomes but lacks CRISPR arrays.
  • A tetracycline resistance gene was identified despite in-vitro susceptibility, and the catalase gene is impaired.
  • In-silico analysis suggests S. aureus subsp. anaerobius evolved as a distinct clone, potentially linked to host adaptation and missing functions.

Conclusions:

  • The genome sequence provides insights into the unique biology of Staphylococcus aureus subsp. anaerobius.
  • Genomic data supports its classification as a distinct clone, separated from other S. aureus strains.
  • Availability of this genome facilitates the development of diagnostic tools for differentiating it from S. aureus.