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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Updated: May 3, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Biosensor-based microRNA detection: techniques, design, performance, and challenges.

Blake N Johnson¹, Raj Mutharasan

¹Department of Chemical and Biological Engineering, Drexel University, Philadelphia, PA 19104, USA. mutharasan@drexel.edu.

|February 7, 2014

Summary

This summary is machine-generated.

This review highlights amplification-free microRNA (miRNA) biosensor techniques, offering femtomolar to attomolar detection limits and rapid results. These biosensors complement traditional molecular techniques for enhanced miRNA analysis.

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Last Updated: May 3, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Area of Science:

Biomedical Engineering
Molecular Diagnostics
Analytical Chemistry

Background:

MicroRNA (miRNA) detection is crucial for disease diagnostics.
Current molecular techniques often require amplification, increasing complexity and time.
Biosensor-based techniques offer a promising alternative for direct miRNA detection.

Purpose of the Study:

To critically review amplification-free biosensor-based techniques for microRNA detection.
To compare biosensors with traditional amplification-based molecular techniques.
To identify current trends and future directions in miRNA biosensor development.

Main Methods:

Review of electrochemical, electromechanical, and optical miRNA biosensor classes.
Analysis of transduction mechanisms, protocols, and sensitivity.
Discussion of challenges in miRNA hybridization thermodynamics and assay bias.

Main Results:

Biosensor techniques demonstrate femtomolar (fM) to attomolar (aM) limits of detection (LOD).
Key advantages include amplification-free detection, short time-to-results (TTR), and multiplexing potential.
Biosensors require minimal sample preparation compared to traditional methods.

Conclusions:

Amplification-free miRNA biosensors are poised to complement existing molecular techniques.
Future development should focus on enhancing measurement confidence and multiplexing capabilities.
Biosensor technology offers a sensitive, rapid, and simplified approach to miRNA analysis.