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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Cellular analysis and detection using surface plasmon resonance techniques

Pegah N Abadian¹, Chase P Kelley, Edgar D Goluch

¹Department of Chemical Engineering, Northeastern University , Boston, Massachusetts 02115, United States.

Analytical Chemistry

|February 8, 2014

Summary

No abstract available in PubMed .

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