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Sphingosine kinase 1 regulates measles virus replication

Madhuvanthi Vijayan1, Young-Jin Seo1, Curtis John Pritzl1

  • 1Departments of Surgery & Molecular Microbiology and Immunology, University of Missouri-Columbia, Columbia, MO 65212, USA.

Virology
|February 8, 2014
PubMed

Insights

Sphingosine kinase (SK) inhibition restricts measles virus (MV) replication by impacting viral protein synthesis. This study reveals SK as a critical host factor essential for efficient MV propagation and NF-κB pathway activation.

Area of Science:

  • Virology
  • Cell Biology
  • Biochemistry

Background:

  • Measles virus (MV) relies on host factors for replication.
  • Sphingosine kinase (SK) regulates cellular processes, including host defense.

Purpose of the Study:

  • To investigate the role of Sphingosine Kinase 1 (SK1) in measles virus replication.
  • To determine if SK inhibition affects MV propagation and associated signaling pathways.

Main Methods:

  • Overexpression and inhibition of SK1 in cells expressing MV receptors (SLAM, Nectin-4).
  • Assessed viral protein expression and infectious virus production.
  • Investigated the impact of SK inhibition on NF-κB signaling pathway activation.

Main Results:

  • SK1 overexpression enhanced MV replication.
  • SK inhibition impaired viral protein expression and infectious virus production across different MV strains.
  • SK inhibition suppressed MV-induced NF-κB activation.
  • NF-κB pathway inhibition also repressed MV protein synthesis.

Conclusions:

  • Sphingosine kinase (SK) is a crucial host factor for efficient measles virus replication.
  • SK inhibition restricts MV replication, partly by modulating the NF-κB signaling pathway.
  • Targeting SK represents a potential strategy for antiviral therapies against measles.
Keywords:
Measles virusNF-κBSphingosine kinase

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