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Related Experiment Videos

Structure and function of SV40 large-T antigen.

S E Mole1, J V Gannon, M J Ford

  • 1Molecular Immunochemistry Laboratory, Imperial Cancer Research Fund, Hertfordshire, U.K.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|December 15, 1987
PubMed
Summary

Simian virus 40 (SV40) large-T antigen, crucial for DNA replication and cell transformation, was studied using functional domains expressed in E. coli and monoclonal antibodies to map its activities.

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Area of Science:

  • Molecular Biology
  • Virology
  • Oncology

Background:

  • Simian virus 40 (SV40) is a small DNA tumor virus extensively used as a model system.
  • SV40 large-T antigen (large-T) is vital for viral DNA replication and cellular transformation.
  • large-T is a multifunctional protein requiring detailed functional domain analysis.

Purpose of the Study:

  • To precisely delineate the functional domains of SV40 large-T antigen.
  • To understand its roles in viral DNA replication.
  • To elucidate its mechanisms in SV40-mediated cellular transformation.

Main Methods:

  • Expression of small functional domains of large-T antigen in Escherichia coli.
  • Analysis using an extensive library of anti-T monoclonal antibodies.

Related Experiment Videos

  • Biochemical and functional assays to characterize protein domains.

    • Main Results:

    • Identification of specific domains within large-T antigen responsible for distinct functions.
    • Characterization of interactions between large-T domains and host cell machinery.
    • Correlation of specific large-T domains with viral replication initiation and transformation.

    Conclusions:

    • The study successfully mapped functional domains of SV40 large-T antigen.
    • This provides a deeper understanding of viral DNA replication and cellular transformation mechanisms.
    • The findings contribute to the utility of SV40 as a model system in molecular biology and cancer research.